PCR (Polymerase Chain Reaction)
Join The Amoeba Sisters as they explain the biotechnology technique PCR. This video goes into the basics of how PCR works as well as two examples of its potential use.
Table of Contents:
00:00 Intro
1:34 How does PCR work?
4:31 Why use PCR?
5:10 rRT-PCR testing for SARS-CoV-2 (virus that causes COVID-19)
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Factual References (and excellent for learning more detail about this topic):
Clark, Mary Ann, et al. “17.1 Biotechnology - Biology 2e | OpenStax.” Biology 2e, OpenStax, 28 Mar. 2018, openstax.org/books/biology-2e/pages/17-1-biotechnology
https://www.fda.gov/consumers/consumer-updates/coronavirus-testing-basics
https://www.fda.gov/media/136151/download
*General length of a primer in base pairs from NIH https://www.genome.gov/genetics-glossary/Primer
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FURTHER READING SUGGESTIONS:
On RT-PCR for SARS-CoV-2:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197457/
https://jamanetwork.com/journals/jama/fullarticle/2765837
On Fluorescent Probes in PCR:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC52277/?page=1
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148886/
What about limitations or disadvantages of PCR?
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4102308/
What about more uses for PCR (as well as more info about Nobel Prize):
https://www.nobelprize.org/prizes/chemistry/1993/press-release/
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The Amoeba Sisters videos demystify science with humor and relevance. The videos center on Pinky's certification and experience in teaching biology at the high school level. Amoeba Sisters videos only cover concepts that Pinky is certified to teach, and they focus on her specialty: secondary life science. Learn more about our videos here: https://www.amoebasisters.com/our-videos
Support Us? https://www.amoebasisters.com/support-us.html
Our Resources:
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GIFs: https://www.amoebasisters.com/gifs.html
Handouts: https://www.amoebasisters.com/handouts.html
Comics: https://www.amoebasisters.com/parameciumparlorcomics
Unlectured Series: https://www.amoebasisters.com/unlectured
Connect with us!
Website: http://www.AmoebaSisters.com
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TIPS FOR VIEWING EDU YOUTUBE VIDEOS:
Want to learn tips for viewing edu YouTube videos including changing the speed, language, viewing the transcript, etc? https://www.amoebasisters.com/pinkys-ed-tech-favorites/10-youtube-tips-from-an-edu-youtuber-duo
MUSIC:
Our intro music designed and performed by Jeremiah Cheshire.
End music in this video is listed free to use/no attribution required from the YouTube audio library https://www.youtube.com/audiolibrary/music?feature=blog
COMMUNITY:
We take pride in our AWESOME community, and we welcome feedback and discussion. However, please remember that this is an education channel. See YouTube's community guidelines and how YouTube handles comments that are reported by the community. We also reserve the right to remove comments.
TRANSLATIONS:
Thank you so much to our amazing translators!
Turkish subtitles by Kardelen Ünalleylioğlu
Hindi subtitles by Alisha Aggarwal
While we don't allow dubbing of our videos, we do gladly accept subtitle translations from our community. Some translated subtitles on our videos were translated by the community using YouTube's community-contributed subtitle feature. After the feature was discontinued by YouTube, we have another option for submitting translated subtitles here: https://www.amoebasisters.com/pinkys-ed-tech-favorites/community-contributed-subtitles We want to thank our amazing community for the generosity of their time in continuing to create translated subtitles. If you have a concern about community contributed contributions, please contact us.
Join The Amoeba Sisters as they explain the biotechnology technique PCR. This video goes into the basics of how PCR works as well as two examples of its potential use.
Table of Contents:
00:00 Intro
1:34 How does PCR work?
4:31 Why use PCR?
5:10 rRT-PCR testing for SARS-CoV-2 (virus that causes COVID-19)
————————————————————————————
Factual References (and excellent for learning more detail about this topic):
Clark, Mary Ann, et al. “17.1 Biotechnology – Biology 2e | OpenStax.” Biology 2e, OpenStax, 28 Mar. 2018, openstax.org/books/biology-2e/pages/17-1-biotechnology
https://www.fda.gov/consumers/consumer-updates/coronavirus-testing-basics
https://www.fda.gov/media/136151/download
*General length of a primer in base pairs from NIH https://www.genome.gov/genetics-glossary/Primer
————————————————————————————
FURTHER READING SUGGESTIONS:
On RT-PCR for SARS-CoV-2:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197457/
https://jamanetwork.com/journals/jama/fullarticle/2765837
On Fluorescent Probes in PCR:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC52277/?page=1
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148886/
What about limitations or disadvantages of PCR?
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4102308/
What about more uses for PCR (as well as more info about Nobel Prize):
https://www.nobelprize.org/prizes/chemistry/1993/press-release/
————————————————————————————
The Amoeba Sisters videos demystify science with humor and relevance. The videos center on Pinky’s certification and experience in teaching biology at the high school level. Amoeba Sisters videos only cover concepts that Pinky is certified to teach, and they focus on her specialty: secondary life science. Learn more about our videos here: https://www.amoebasisters.com/our-videos
Support Us? https://www.amoebasisters.com/support-us.html
Our Resources:
Biology Playlist: https://www.youtube.com/playlist?list=PLwL0Myd7Dk1F0iQPGrjehze3eDpco1eVz
GIFs: https://www.amoebasisters.com/gifs.html
Handouts: https://www.amoebasisters.com/handouts.html
Comics: https://www.amoebasisters.com/parameciumparlorcomics
Unlectured Series: https://www.amoebasisters.com/unlectured
Connect with us!
Website: http://www.AmoebaSisters.com
Twitter: http://www.twitter.com/AmoebaSisters
Facebook: http://www.facebook.com/AmoebaSisters
Tumblr: http://www.amoebasisters.tumblr.com
Pinterest: http://www.pinterest.com/AmoebaSisters
Instagram: https://www.instagram.com/amoebasistersofficial/
Visit our Redbubble store at https://www.amoebasisters.com/store
TIPS FOR VIEWING EDU YOUTUBE VIDEOS:
Want to learn tips for viewing edu YouTube videos including changing the speed, language, viewing the transcript, etc? https://www.amoebasisters.com/pinkys-ed-tech-favorites/10-youtube-tips-from-an-edu-youtuber-duo
MUSIC:
Our intro music designed and performed by Jeremiah Cheshire.
End music in this video is listed free to use/no attribution required from the YouTube audio library https://www.youtube.com/audiolibrary/music?feature=blog
COMMUNITY:
We take pride in our AWESOME community, and we welcome feedback and discussion. However, please remember that this is an education channel. See YouTube’s community guidelines and how YouTube handles comments that are reported by the community. We also reserve the right to remove comments.
TRANSLATIONS:
Thank you so much to our amazing translators!
Turkish subtitles by Kardelen Ünalleylioğlu
Hindi subtitles by Alisha Aggarwal
While we don’t allow dubbing of our videos, we do gladly accept subtitle translations from our community. Some translated subtitles on our videos were translated by the community using YouTube’s community-contributed subtitle feature. After the feature was discontinued by YouTube, we have another option for submitting translated subtitles here: https://www.amoebasisters.com/pinkys-ed-tech-favorites/community-contributed-subtitles We want to thank our amazing community for the generosity of their time in continuing to create translated subtitles. If you have a concern about community contributed contributions, please contact us.
