Rick van de Zedde & Pieter de Visser, Wageningen University & Research. At Wageningen University & Research from April 2021 onwards, a digital twin (DT) will be operational. The DT will digitally represent a tomato crop of individual, virtual plants in their local greenhouse environment, and grown simultaneously. The DT will feature real-time updating of plant parameters and environmental variables based on high-tech sensor equipment available in the Netherlands Plant Eco-phenotyping Centre (NPEC) facilities. In the DT, each tomato plant in the crop will be modelled in 3D integrating a set of traits that correspond to model parameters. Thereby, the DT enables us to predict crop response (growth, development and production) to greenhouse and management conditions that affect production efficiency; light intensity and quality, CO2 dosing, nutrient availability and leaf pruning. Thus, the DT can support greenhouse management in real-time. This will be the first-ever 3D simulation model of individual plants growing in greenhouses that get updated by sensor data and that delivers updated predictions as the real plants grow. In that sense, it is a true digital twin, which does not yet exist for plants. This is an important extension of the plant and greenhouse modelling that exists today. As well, the DT allows for hypothesis testing and in silico experiments. As a scientific aim, we will develop and study novel methods on e.g. deep learning for processing of sensor data to transform the raw data to plant traits. Moreover, novel methods will be dealt with on Bayesian inference [More]